Basic Molecular Biology Module 4: PCR and Real-Time PCR

Description

Molecular techniques have been widely used in clinical diagnosis, e.g., diagnosing disease, predicting disease course, and identifying infectious agents. This basic molecular biology course series will introduce the scientific background for molecular diagnosis, the principles of molecular biology laboratory practice, and common methods.

This basic-level eLearning course provides information on the principle of PCR and real-time PCR. Topics covered include PCR steps, PCR product analysis, and real-time PCR characteristics.

Audience

This online course is designed for public health and clinical laboratory staff, and persons interested in PCR and real-time PCR techniques.

Objectives

At the conclusion of this course, the participant will be able to:

• Explain the basic steps involved in PCR
• Identify the components of PCR, reverse transcription PCR, and PCR product analysis
• Recognize the characteristics of real-time PCR
• Identify the techniques used to detect products in real-time PCR
• Differentiate the nucleic acid quantification processes used in real-time PCR
• Explain the roles of PCR controls