Basic Molecular Biology Module 3: Nucleic Acid Extraction

Description

Molecular techniques have been widely used in clinical diagnosis, e.g., diagnosing disease, predicting disease course, and identifying infectious agents. This basic Molecular Biology course series will introduce the scientific background for molecular diagnosis, the principles of molecular biology laboratory practice, and common methods.

This basic-level eLearning course, Module 3, provides information on nucleic acid extraction. Topics covered include extraction method selection, basic extraction steps, and nucleic acid analysis.

Audience

This online course is designed for public health and clinical laboratory staff and persons interested in nucleic acid extraction.

Objectives

At the conclusion of this course, the participant will be able to:

• Identify the four major factors used in selection of the nucleic acid extraction method
• Outline the three basic steps in nucleic acid extraction
• Explain how to analyze nucleic acid quantity and purity by spectrophotometry and gel electrophoresis
• Identify common problems in nucleic acid extraction